Comparison of intracellular cytokine production with extracellular cytokine levels using two flow cytometric techniques
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چکیده
منابع مشابه
Flow-cytometric assessment of lymphocyte cytokine production in tuberculosis.
We assessed by flow-cytometry the Th1/Th2 profiles in peripheral blood lymphocytes (PBL) from patients with active tuberculosis (TB), before and after antituberculous therapy, and from healthy tuberculin-positive and -negative reactors. PBL from patients showed a reduced potential for Th1-cytokine (notably IFN- gamma) production after culture with a policlonal stimulus. When these PBL from pati...
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A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination of their CD45-positivity and their typical side scatter properties. The identity of the CD45-posit...
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چکیده ندارد.
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Over the last twenty years, the debilitating clinical condition known as sepsis has tripled in frequency to become the tenth leading cause of death in the United States. Extracellular histones were previously reported to mediate cell damage and organ dysfunction during hyperinflammatory response, a characteristic feature of sepsis. Lipopolysaccharide (LPS) is an endotoxin found in Gramnegative ...
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Background: Although many experimental studies provide convincing evidence that type II immunity is protective against helminths, recent data in mice demonstrate that Th1 is also impor-tant in some cestodes like Hymenolepis nana. Objective: To identify the role of Th1 and Th2 lymphocytes in immunity against H. nana, the levels of IL-12, IFNγ, IL-5, and IL-13 were de-termined in serum of humans ...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2003
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.b.10041